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LigaFast Rapid DNA Ligation System
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LigaFast Rapid DNA Ligation System
LigaFast Rapid DNA Ligation System
LigaFast Rapid DNA Ligation System from Promega
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Citations:
(19)
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LigaFast Rapid DNA Ligation System
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Description
T4 DNA Ligase and buffer combination for rapid (5-minute) ligations
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
LigaFast Rapid DNA Ligation System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
M8221
Time
Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature.
Quantity
30 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (19)
(1)
Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
STAR Protocols
March 1, 2023
Angela Rose Liu, Shrikanth C. Basavarajappa, Nandini Sarkar, Anna Bruchez, Parameswaran Ramakrishnan
Glutaraldehyde,Sigma,354400 Xho1,
NEB
,
R0146L
Xba1,
NEB
,
R0145L
HindIII
,
NEB
,
R0104L
Agarose,Fisher,BP160-500
Ethidium bromide
,
Sigma
,E8751
Ligafast Rapid DNA Ligation System
,
Promega
,
M8221
2× Rapid
DNA ligation
buffer,Promega,
M8221
Ampicillin,Fisher,BP176025 CaCl2,Sigma,C1016 Na2HPO4,
Sigma
,S3264
HEPES
,
Santa Cruz Biotechnology
(2)
Protocol to analyze lipid asymmetry in the plasma membrane
STAR Protocols
December 16, 2022
Yugo Miyata, Katsumori Segawa
Kit,
QIAGEN
,
27104
Wizard SV Gel and PCR Clean-Up System
,
Promega
,
A9281
LigaFast Rapid DNA Ligation System
,
Promega
,
M8221
"Millex-GV Syringe Filter Unit, 0.22 μm, PVDF",MERCK,SLGVR33RS Xpress Micro Dialyzer MD300 12–14 kDa,Scienova,40790 5
(3)
KLF4 recruits SWI/SNF to increase chromatin accessibility and reprogram the endothelial enhancer landscape under laminar shear stress
Nature Communications
August 23, 2022
Jan-Renier Moonen et al.
Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated using LigaFast (#
M8221
,
Promega
) and size-selected by gel extraction before
PCR amplification
. The purified libraries were
(4)
Disruption of RING and PHD Domains of TRIM28 Evokes Differentiation in Human iPSCs
Cells
July 29, 2021
Sylwia Mazurek, Urszula Oleksiewicz, Patrycja Czerwińska, Joanna Wróblewska, Marta Klimczak, Maciej Wiznerowicz
pGEM-T Easy vector (#
A1360
,
Promega
, Madison, WI, USA) with a
LigaFast Rapid DNA Ligation System
(#
M8221
,
Promega
, Madison, WI, USA) and cloned into
E. coli
DH5a (#
T3007
,
Zymo Research
, Irvine, CA, USA). The
(5)
Direct analysis of brain phenotypes via neural blastocyst complementation
Nature Protocols
October 1, 2020
Hai-Qiang Dai, Zhuoyi Liang, Amelia N. Chang, Aimee M. Chapdelaine-Williams, Beatriz Alvarado, Alex A. Pollen, Frederick W. Alt, Bjoern Schwer
M0201S
)
LigaFast Rapid DNA Ligation System
(contains
T4 DNA ligase
and 2× Rapid
ligation
buffer,
Promega
, cat. no.
M8221
) 10 mM ATP (
NEB
, cat. no.
P0756S
) Plasmid-Safe DNAse and 10× PlasmidSafe Buffer (Lucigen, cat. no. E3101K)
Taq DNA Polymerase (250 U
(6)
CRISPR/Cas9-mediated genome editing in mouse embryonic stem cells and direct analysis of brain phenotypes via neural blastocyst complementation
Protocol Exchange
October 19, 2018
Frederick W. Alt, Bjoern Schwer, Amelia N. Chang, Zhuoyi Liang, Hai-Qiang Dai, Aimee M. Chapdelaine-Williams, Frederick W. Alt
LigaFast Rapid DNA Ligation System
\(contains
T4 DNA ligase
and
2x Rapid ligation buffer
,
Promega
, cat. no.
M8221
) 10 mM ATP \(
NEB
, cat. no.
P0756S
) Plasmid-Safe DNAse and 10x PlasmidSafe Buffer \(Lucigen, cat. no.
(7)
Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosis
Cell reports
October 2, 2018
Turapov O, Forti F, Kadhim B, Ghisotti D, Sassine J, Straatman-Iwanowska A, Bottrill AR, Moynihan PJ, Wallis R, Barthe P,
et al
.
Ltd- /,Cat #
R3193S
;
R3182L
;
R0111L
;
R3136L
;
R3142L
LigaFast Rapid DNA Ligation System
,
Promega
,Cat#
M8221
SignalFire Elite ECL Reagent
,
Cell Signaling Technology
,Cat#
12757S
Titansphere Phos-TiO Kit,GL Sciences,Cat#
(8)
Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy
Methods in Molecular Biology
March 7, 2018
Scot J. Matkovich, Gerald W. Dorn
M0212S
), 1 mM
dATP solution
in aliquots,
Promega
LigaFast
T4 DNA ligase
and reaction buffer (catalog #
M8221
). 2% low-melt agarose (e.g. Amresco Agarose II) to prepare gels for library size selection. Indexing
(9)
Histone variant H2A.J accumulates in senescent cells and promotes inflammatory gene expression
Nature communications
May 10, 2017
Contrepois K, Coudereau C, Benayoun BA, Schuler N, Roux PF, Bischof O, Courbeyrette R, Carvalho C, Thuret JY, Ma Z,
et al
.
#
M0212s
, Ipswich, MA) and dATP, Illumina adapters were ligated to the protruding 3′-‘A' base (
Promega
Cat #
M8221
, Madison, WI). Ligated products were size selected on 2% E-gel (250-300 bp). The
dUTP
-containing second
(10)
DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes
Nucleic Acids Research
June 20, 2016
Si Ho Choi, Micah D. Gearhart, Ziyou Cui, Darko Bosnakovski, Minjee Kim, Natalie Schennum, Michael Kyba
Scientific, 514103) were ligated to DNA fragments using
LigaFast™ Rapid DNA Ligation System
(
Promega
,
M8221
) and 200–500 base pair DNA was selected using Agencourt AMPpure XP (
Beckman Coulter
,
A63881
). 15 cycles
(11)
Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform
Cold Spring Harbor Protocols
July 1, 2015
Rui Chen, Hogune Im, Michael Snyder
exo-) with NEB Buffer #2 (
New England BioLabs
M0212s
)
LigaFast Rapid DNA Ligation System
(
Promega
M8221
) MinElute PCR Purification Kit (
QIAGEN
28006
) Nuclease-free water (UltraPure
Distilled Water
[
Life Technologies
(12)
Whole-Exome Enrichment with the Roche NimbleGen SeqCap EZ Exome Library SR Platform
Cold Spring Harbor Protocols
July 1, 2015
Rui Chen, Hogune Im, Michael Snyder
(3′→5′ exo-) with NEB Buffer #2 (
New England BioLabs
M0212S
)
LigaFast Rapid DNA Ligation System
(
Promega
M8221
) MinElute PCR Purification Kit (
QIAGEN
28006
)
Nimblegen SeqCap
EZ
Human
Exome Library v2.0 (
Roche
, 05
(13)
Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates
BMC Genomics
December 1, 2014
Habib A Shojaei Saadi, Christian Vigneault, Mehdi Sargolzaei, Dominic Gagné, Éric Fournier, Béatrice de Montera, Jacques Chesnais, Patrick Blondin, Claude Robert
modifications. To optimize the protocol, different ligases including
LigaFast Rapid DNA Ligation System
(#
M8221
,
Promega
, Madison, WI, USA) and
ExpressLink T4 DNA Ligase
(#
A13726
,
Invitrogen
, Carlsbad, CA, USA) were
(14)
Using ChIP-seq Technology to Identify Targets of Zinc Finger Transcription Factors
Methods in Molecular Biology
August 26, 2014
Henriette O’Geen, Seth Frietze, Peggy J. Farnham
5′-exo-minus) (5,000 U/mL;
NEB
#
M0212S
). 3. 100 mM
dATP
. 4. LigaFast DNA ligase (3 U/μL;
Promega
#
M8221
). 5. Oligo only kit for single end read
sequencing
containing genomic adapter oligo mix (100 μM) and
(15)
High-resolution mapping of open chromatin in the rice genome
Genome Research
January 1, 2012
Wenli Zhang, Yufeng Wu, James C. Schnable, Zixian Zeng, Michael Freeling, Gregory E. Crawford, Jiming Jiang
Linker for single read was ligated to the DNA molecules with dA overhang using LigaFast kit (
Promega
,
M8221
). After 2% agarose gel running with TAE buffer, the gel region containing ;150–300 bp DNA was excised
(16)
Diverse Roles and Interactions of the SWI/SNF Chromatin Remodeling Complex Revealed Using Global Approaches
PLoS Genetics
March 3, 2011
Ghia M. Euskirchen, Raymond K. Auerbach, Eugene Davidov, Tara A. Gianoulis, Guoneng Zhong, Joel Rozowsky, Nitin Bhardwaj, Mark B. Gerstein, Michael Snyder
which have a single ‘T’ base overhang at the 3′ end. After adapter
ligation
(LigaFast,
Promega
Cat#
M8221
) DNA was
PCR
-amplified with Illumina genomic DNA primers 1.1 and 2.1 for 15 cycles by using a program
(17)
Inducible production of recombinant human Flt3 ectodomain variants in mammalian cells and preliminary crystallographic analysis of Flt3 ligand-receptor complexes
Acta crystallographica. Section F, Structural biology and crystallization communications
March 1, 2011
Verstraete K, Remmerie B, Elegheert J, Lintermans B, Haegeman G, Vanhoenacker P, Van Craenenbroeck K, Savvides SN.
were ligated into the expression vectors using the LigaFast
ligation
system (
Promega
, catalogue No.
M8221
). The final expression constructs were sequence-verified (Cogenics, Meylan, France) and matched the
human
(18)
Deep mRNA sequencing for in vivo functional analysis of cardiac transcriptional regulators: application to Galphaq
Circulation Research
May 14, 2010
Scot J. Matkovich, Yan Zhang, Derek J. Van Booven, Gerald W. Dorn
three nt ‘barcodes’ were ligated to the cDNA at 10:1 molar excess using the
Promega
LigaFast kit (#
M8221
); different barcoded adapters were ligated to individual
mouse
heart cDNAs. Following column-
purification
(19)
Genome-Wide Identification of Binding Sites Defines Distinct Functions for Caenorhabditis elegans PHA-4/FOXA in Development and Environmental Response
PLoS Genetics
February 19, 2010
Mei Zhong et al.
ChIP DNA was then ligated with the adapter mix from the Illumina kit using LigaFast from
Promega
(Cat#
M8221
). The DNA was then purified using the QIAquick PCR Purification Kit and protocol between each step.
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LigaFast Rapid DNA Ligation System from Promega
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